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KMID : 0613820130230060779
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Journal of Life Science
2013 Volume.23 No. 6 p.779 ~ p.788
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Effect of High-fat Diet on Peritoneal Macrophage Immunocompetence in C57/BL6 Mice
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Jeong Jun-Hyun
Park Hee-Geun
Lee Wang-Lok
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Abstract
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This study was to investigate the effect of high-fat diet on macrophage immunocompetence in C57BL/6 mice. C57BL/6 male mice (4 weeks aged, n=16) were divided into two groups. HD groups fed high-fat diet (45% of fat) and ND groups fed chow diet (10% of fat). Peritoneal macrophages were obtained from each mouse intra-peritoneal by sterile lavage method. Macrophage were stimulated with 1μg/ml of lipopolysaccharide (LPS) for 24 hr. Body weight was significantly increased by high-fat diet. Macrophage phagocytosis of HD was significantly lower than that of ND. After 24 hr of LPS stimulation, NO, IL-1β and IFN-r production of HD were significantly lower than those of ND. There were no significant differences in the production of TNF-α and IL-12 between HD and ND. These findings suggest that high fat diet-induced obesity is associated with decreased Immunocompetence and antigen-stimulated sensitivity of peritoneal macrophage, and lower production of NO, IL-1β and IFN-r may contribute to these changes.
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KEYWORD
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Cytokine, high fat diet, macrophage, nitric oxide, phagocytosis
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